Synthesis and enzymatic properties of deoxyribooligonucleotides containing methyl and phenylphosphonate linkages.
نویسندگان
چکیده
Chemical methods for the synthesis of short deoxyribooligonucleotides containing methyl and phenylphosphonodiester linkages have been developed. The interaction of two such nonionic dinucleotide analogs, T(pCH3)T and T(pC6H5)T, with several enzymes has been investigated. Because of the phosphonate linkage each dinucleotide exists as a diastereomeric pair as shown by thin layer chromatography and enzymatic studies. Both isomers of each dinucleotide can be phosphorylated by T4-polynucleotide kinase in the presence of [gamma-32P]ATP. Only one of the diastereoisomers of each dinucleotide is slowly hydrolyzed by snake venom phosphodiesterase and acts as an inhibitor of the enzyme-catalyzed hydrolysis of 5'-labeled oligothymidylic acid. Both isomers of each dinucleotide analog are completely resistant to hydrolysis by spleen phosphodiesterase.
منابع مشابه
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.
The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and...
متن کاملNew Thermally Stable Aromatic Polyimides Based on Aromatic Diamine 2,5-Bis(3-amino-4-methyl benzene)-1,3,4-oxadiazole (BAMO): Synthesis and Characterization
In current study, the synthesis and characterization of novel thermally stable polyimides (PIs) containing an 1,3,4-oxadiazole moiety based on a diamine, i.e. 2,5-bis(3-amino-4-methyl benzene)-1,3,4-oxadiazole (BAMO), have been reported. The polymers were characterized using FT-IR and elemental analysis (CHN). Thermal and mechanical behaviours of the prepared PIs were studied by thermo-gravimet...
متن کاملSynthesis, characterization and polymerization of a novel acrylate monomer containing both 4H-pyran-4-one and 1,2,3-triazole moiety and evaluation of their antibacterial activity
A novel acrylate monomer containing 4H-pyran-4-one and 1,2,3-triazole ring, {1-[4-(4-oxo-6-phenyl-4H-pyran-2-yl)benzyl]-1,2,3-triazol-4-yl}methyl acrylate was synthesized by the reaction of 2-{4-[(4-(hydroxymethyl)-1,2,3-triazol-1-yl)methyl]phenyl}-6-phenyl-4H-pyran-4-one with acryloyl chloride in the presence of triethylamine. The structure of the acrylate monomer was established on the basis ...
متن کاملPreparation and Properties of Thermally Stable Polyureas Containing Ether and Ketone Units
The main objective of this search was to prepare novel soluble polyureas with improved thermal stability. Accordingly, a new types of polyureas was prepared through the polycondensation reaction of a prepared diamine containing ether, keto, and naphthyl groups with 4,4’-diphenylmethan diisocyanate (MDI), toluene-2,4-diisocyanate (TDI), and isophorone diisocyanate (IPDI) in N-methyl-2-pyrrol...
متن کاملThermally Stable Poly(amide-imide)/Nanocomposites Reinforced Silicate Nanoparticles Containing Bicyclo Segment and Dibenzalacetone Moiety: Synthesis and Characterization
Two new samples of poly(amide-imide)/nanocomposites containing bicyclo segment and dibenzalaceton moiety in the main chain were synthesized by a solution intercalation technique. Poly(amide-imide) (PAI) 8 as a source of polymer matrix was synthesized by the direct polycondensation reaction of N,N´-bis(4-carboxyphenyl)bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetracarboxylic diimide 3 with 2,6-bis(4-amino...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nucleic acids research
دوره 6 9 شماره
صفحات -
تاریخ انتشار 1979